IVIS System

Bioluminescent ImagingBioluminescent Imaging

 

Bioluminescent Imaging :Optical Imaging Equipment

  • Bioluminescence refers to the process of visible light emission in living organisms.
  • Bioluminescent imaging is a methodology that has been developed over the last several years as a tool for molecular imaging of small laboratory animals.
  • This form of optical imaging is noninvasive and facilitates real-time analysis of disease processes in living organisms

 

Photons Diffuse Through Tissue

Imaging Illustration
  •   Light traveling through tissue scatters many times creating a “fuzzy” image at the surface of the animal
  •   Optical imaging captures the diffuse image on the surface of the subject
  •   Surface intensity depends on:
    • Source depth and brightness
    • Wavelength
    • Tissue optical properties

 

 

 


Optical Imaging Reporters

 

Example of Luciferin Kinetics

Luciferin Kinetics Graph
Luciferin Kinetics exampleLuciferin Kinetics results

Optical Imaging Window

optimagewindow

In Vivo Imaging Reduces Animal Usage

Current Methodology = 24 animals over four treatment points

animal use graphic 1

BPI Methodology = the same 6 animals over four treatment points

Animal usage graphic 2

Same group of anesthetized test animals at each time point of an experiment uses far fewer animals than current methodology

Imaging Applications

  • Oncology
  • Infectious Diseases
  • Inflammation
  • Apoptosis
  • Stem Cells
  • Gene Therapy
  • Gene Expression In Vivo
    -Oncology/Angiogenesis LPTA Models
    - Inflammation LPTA Models

In Vitro:

  • HTS Screening
  • Colony Differentiation
  • Molecular Biology
InVitro illustration

 

 

 

Ex Vivo:

  • Histological Slides
  • Organ Analysis
histoorgan

 

 

 

Beyond Small Animals

  • Botanical Models
  • Insects
  • Monkeys, Cats, etc.
Beyond Small Animals image

 

 

 

 

 

 

 

 

3500+ Publications in Peer Review Journals

Journal Images

Graph Image
Orthotopic Prostate Model
Spontaneous Pancreatic Cancer Model
Metastasis Model
Glioma Model

 

Summary

  • Bioluminescent imaging provides a non-invasive means of following disease progression (same animal used for all time points means fewer animals needed per study)
  • Tumor mass correlates with bioluminescent signal
  • Good tissue penetration (organs, bone)
  • Wide range of applications for drug discovery and development