Passive Cutaneous Anaphylaxis (PCA)

Passive cutaneous anaphylaxis (PCA) is an immediate dermal response to an allergen-IgE interaction and is characterized by increased permeability of vessels within the skin. To visualize and quantify the increased permeability characteristic of PCA, the intravenous injection of Evans blue, a dye that binds to and is extravated with plasma albumin, is utilized. PCA can be used as a tool to study sensitivity to allergens and compound efficacy in ameliorating these responses.

Category(s)

Preclinical Drug Efficacy Studies

Species

Mice

Basic Methodology

One ear of the animal is injected with Anti-DNP-IgE, and the other is left alone. 1-hour post-dosing animals are given a tail vein injection of Evan’s blue dye followed by a second tail vein injection of DNP-HSA. Signs of blue reaction and swelling are noted. 1-hour after the last injection, mice are euthanized and ear punch biopsies from both ears are harvested to quantify Evan’s blue content in the ear tissue. The administration of therapeutic compounds is performed according to the schedule set-forth in the study protocol.

Measurements

Clinical observations, body weight, quantification of PCA (Evan’s blue dye spectroanalysis), and cytokine profiling using ELISA and Luminex multiplex assays

Reference Substance(s):

Cyproheptadine

Literature References:

Bae EA, Park EK, Yang HJ, Baek NI and Kim DH. (2006) Hinokiresino Inhibits IgE-induced Mouse Passive Cutaneous Anaphylaxis Reaction. Planta Medica; 72: 1328-30.

Ovary Z. (1958) Passive Cutaneous Anaphylaxis in the Mouse. The Journal of Immunology; 81: 355-57.

Saria A and Lundberg JM. (1982) Evans blue fluorescence: quantitative and morphological evaluation of vascular permeability in animal tissues. Journal of Neuroscience Methods; 8: 41-9.