Murine Skin Transplantation (Allograft)
Host acceptance of donor tissue is dependent upon MHC compatibility and host rejection is largely dependent on T-cell response to non-MHC compatible tissues. The transplantation of skin from a donor to a host (allograft) is a stringent method to assay the T-cell responses of the host to donor MHC-disparate antigens. The murine skin transplantation model is useful for the assay of host T-cell responses to donor antigens (tissues) and for investigating the efficacy of novel compounds in reducing these T-cell responses, increasing the level of host acceptance to donor tissue.
Preclinical Drug Efficacy Studies
The skin of the donor animal is harvested and placed into a plate of ice. A small incision is made and skin from the recipient is removed providing a graft bed. The donor skin is placed flatly into the graft bed and bandaged. Eight to twelve days later the bandages are removed. Grafts are monitored daily for signs of rejection. The administration of compounds is performed according to the schedule set-forth in the study protocol.
Clinical observations, macroscopic examination of harvested skin patches, immunohistochemical analysis of skin patches, cytokine profiling using ELISA and Luminex multiplex assays
Jun Yang, Joyce Popoola, Shakila Khandwala Nidyanandh Vadivel, Xueli Yuan, Shirine Dada, Indira Guleria Chaorui Tian M. Javeed Ansari, Tahiro Shin, Hideo Yagita, Miyuki Azuma, Mohamed H. Sayegh, Anil Chandraker. (2008) Critical Role of Donor Tissue Expression of Programmed Death Ligand-1 in Regulating Cardiac Allograft Rejection and Vasculopathy. Circulation; 117: 660-669.
Magae J, Miller MW, Nagai K and Shearer GM. (1996) Effect of Metacycloprodigiosin, an Inhibitor of Killer T Cells, on Murine Skin and Heart Transplants. The Journal of Antibiotics; 49(1): 86-90.