Endotoxin/lipopolysaccharide (LPS) is the major mediator responsible for triggering the cellular and humoral responses of shock induced by Gram-negative bacteria. The toxic responses of LPS are mediated by various cytokines. The LPS murine model is useful in identifying inflammatory molecules involved in endotoxemia and for determining the efficacy of compounds designed to treat this condition.
Preclinical Drug Efficacy Studies
Mice (C57BL/6, Male, 8-10 weeks)
Animals are injected with LPS and observed for 8-hrs post-challenge. Serum is collected for the assay of IL-1, IL-6 and TNF-alpha; as well as CBCs. Dosing is performed according to the schedule set-forth within the study protocol.
Clinical observations, body weight, ELISA and Luminex assays to measure serum concentrations of IL-1, IL-6 and TNF-alpha, CBCs
Kaima R, Matsumoto S, Higashi H, Matsushima K. (1999) The Molecular Pathogenesis of endotoxic shock and organ failure. Molecular Medicine Today; 5(3): 123-32.