Induced Skin Inflammation

Skin damage induced through tape stripping mimics the features of many skin inflammatory conditions including cutaneous lupus, psoriasis, lichen planus and dermatomyositis by disrupting the cutaneous epithelial barrier and stimulating specific inflammatory factors. This model may be used both as a platform to uncover the pathogenic mechanisms of these diseases through the use of diagnostic compounds and/or to determine compound efficacy in disease treatment.

Category(s)

Preclinical Drug Efficacy Studies

Species

Mice (Strain specific to pathway of study, contact us for further information)

Basic Methodology

The dorsal area of the mouse is shaved and tape stripped. Additionally, epicutaneous sensitization with an allergen (ex OVA) may be applied. The administration of therapeutic compounds is performed according to the schedule set-forth in the study protocol.

Measurements

Clinical observations, macroscopic examination of harvested skin patches, histochemical analysis, cytokine profiling using ELISA and Luminex multiplex assays

Literature References:

Jin H, Oyoshi MK, Le Y, Bianchi T, Koduru S. Mathias CB, Kumar L, Bras SL, Young D, Collins M, Grusby MJ, Wenzel J, Bieber T, Boes M, Silberstein LE, Oettgen HC, Geha RS. Il-21R is essential for epicutaneous sensitization and allergic skin inflammation in humans and mice. The Journal of Clinical Investigation; 119(1): 47-60.

Wenzel J, Tuting T (2007) Identification of type 1 interferon-associated inflammation in the pathogenesis of cutaneous lupus erthematosus opens up options for novel therapeutics approaches. Experimental Dermatology; 16(5): 454-463.