IBD is characterized by inflammation in the mucosal lining of the colon. DSS induces IBD through its direct toxic effects on the colonic epithelium which stimulates an inflammatory response in known susceptible mouse strains. It may also stimulate further inflammation through it’s interaction with luminal bacteria. This model is useful for investigating the pathogenesis of inflammatory bowel diseases and in screening novel compounds for their efficacy in treating it.
Preclinical Drug Efficacy Studies
Mice (C57BL/6 and BALB/c)
IBD is induced by administering DSS in acidified drinking water at a concentration of 3.5% for 5-days. The administration of therapeutic compounds is performed over 6-days according to the schedule set-forth in the study protocol.
General clinical observations, clinical scoring of IBD severity, body weight, colon length and weight, colon histopathology, hem occult fecal analysis, fecal consistency, and cytokine profiling using ELISA and Luminex multiplex assays
Egger B, Bajad-Elliot M, MacDonald TT, Inglin R, Eysselien VE, Buchler MW. (2000) Characterization of acute murine dextran sodium sulphate colitis: Cytokine profile and dose dependency. Digestion; 62:240-248.