Granulation tissue is a matrix of a variety of cell types that forms during wound healing. This tissue is the scaffold from which new cells are generated to heal the wounded area. Fibroblasts create and modify this scaffold, and if they proliferate too quickly and collagen is overproduced and deposited, a scar is formed. Chemical signals, such as various growth factors and cytokines, and the time at which they are expressed, are key factors in controlling the amount and type of cellular growth.
By utilizing full thickness incisional and excisional dermal wounds, compound efficacy can be determined after the administration of either a prophylactic or therapeutic treatment through macroscopic and histological examination as well as immunohistochemical analysis of wounds.
Preclinical Drug Efficacy Studies
Murine (Mice or Rats)
Mice or rats are anesthetized with Isoflurane and shaved with clippers. 1-2 cm incisions are made into the skin along the back using aseptic techniques. If the wound requires a suture, sutures are applied to the wound area. Wounds are harvested at study completion. The administration of therapeutic compounds is performed according to the schedule set-forth in the study protocol.
Clinical observations, body weight, macroscopic and histological observations of harvested wounds, immunohistochemical analysis of harvested wounds, serum cytokine profiling using ELISA and Luminex multiplex assays
Kovacs EJ and DiPietro LA. (1994) Fibrogenic Cytokines and Connective Tissue Production. The FASEB Journal; 8: 854-861.
Granstein RD, Deak MR, Jacques SL, Margolis RJ, Flotte TJ, Whitaker D, Long FH and Amento EP. (1989) The System Administration of Gamma Interferon Inhibits Collagen Synthesis and Acute Inflammation in a Murine Skin Wounding Model. Journal of Investigative Dermatology; 93: 18-27.