Collagen Induced Arthritis (CIA)

Collagen induced arthritis (CIA) is an experimental model of human rheumatoid arthritis (RA), an autoimmune disease that is characterized by the attack and degradation of articular cartilage. CIA is induced by immunization with type LL collagen Cll, the major constituent protein of articular cartilage, in a susceptible mouse strain, DBA/1. Following immunization, animals develop an autoimmune-mediated polyarthritis that shares several clinical, histological, and immunological features with the human autoimmune disease rheumatoid arthritis. Collagen-induced arthritis (CIA) is a valuable model for elucidating pathogenic mechanisms and evaluating the therapeutic effects of novel compounds on rheumatoid arthritis.

Category(s)

Preclinical Drug Efficacy Studies

Species

Mice (DBA/1, Male, 6-8 weeks of age)

Basic Methodology

Mice are injected with LL collagen Cll. Following injection mice are observed and scored for clinical manifestations of arthritic disease. Therapeutic compounds are administered via one of several means to study efficacy as set-forth in the study protocol.

Measurements

Clinical scoring of arthritis, body weights, histopathology, cytokine profiling using ELISA and Luminex multiplex assay, and flow cytometric analysis for the characterization of inflammatory cell types

Reference Substance(s):

Dexamethasone

Literature References:

Duris FH, Fava RA, Noelle RJ. (1994) Collagen-Induced Arthritis as a Model of Rheumatoid Arthritis. Clinical Immunology and Immunopathology; 73(1): 11-18.

McIndoe RA, Bohlman B, Chi E, Schuster E, Lindhardt M, Hood L. (1999) Localization of non-Mhc collagen-induced arthritis susceptibility loci in DBAy1j mice. PNAS; 96: 2210-14.