Our scientists are experienced in producing monoclonal antibodies as well as developing and running ELISAs and other immunological assays for research and development. Our expertise in cell biology enables us to develop, validate and perform cell-based assays from primary tissue, such as bone marrow, spleen, liver, blood, as well as with tissue culture cell lines. Oftentimes screening a pharmaceutical agent for efficacy in a disease model requires assaying the levels of specific biomarkers using ELISAs or other bioassays.
Many of our clients develop pharmaceuticals that require cell-based immunoassays to measure an immune response. We can test sera not only for IgG or other antibody isotype responses but can determine whether cell activation is involved, by using cell-based immunoassays such as the mixed lymphocyte reaction (MLR) or natural killer cell activation. For toxicity studies we can determine whether cytotoxic T lymphocytes have been sensitized to your drug or whether the immune system cells have been activated by the detection of released cytokines or the presence of a specific cellular receptor.
Often cell-based immunoassays require the pairing of two antibodies, one for capture and one for detection. Since polyclonal antibody affinities change over time as the T cells mature, an assay employing monoclonal antibodies may result in a more reproducible result. Additionally, affinity purification using a monoclonal antibody will result in reproducible yields. Our scientists will consult with you about producing hybridomas that generate monoclonals with the desired characteristics. We will conjugate your hapten, or if your antigen is a protein, we will suggest an immunization protocol. We will develop your screening assay or implement the assay you’ve developed and screen your initial cell cultures after fusion as well as screen your clones. The conditioned media will be delivered after further screens or after consultation with you. If you desire further characterization of the monoclonal antibodies, our scientists will discuss with you your needs and perform the needed analyses.
ELISA (Enzyme-linked ImmunoSorbent Assay)
Custom ELISA assay development and validation is frequently needed for drug purification, stability or lot release. We can develop your ELISA assay using polyclonal or monoclonal antibodies supplied by you or use the antibodies we develop here at MuriGenics. Our scientists will consult with you about your final use for the ELISA assay and discuss various formats that can be designed into your assay. Should you have an ELISA assay already developed and wish to have our scientists use it to test drug metabolism, drug stability or lot release, we will employ your protocols.
Luminex xMAP System
Understanding the pathogenesis of a disease state and your pharmaceutical’s efficacy in ameliorating the condition requires a large base of knowledge. A comprehensive analysis is usually derived from data obtained through the examination of multiple disease biomarkers. Performing individual ELISAs or other bioassays for each individual marker can become both costly and time consuming. The Luminex xMAP system allows for the measurement of multiple targets within an individual sample. Luminex technology involves color-coding tiny beads, called microsphere, into 100 distinct sets. Each bead set can be coated with a reagent specific to a particular bioassay, allowing the capture and detection of multiple analytes from a single sample. Analytes are available for quantifying the levels of specific proteins or other macromolecules (ELISA) or for gene expression profiling (bDNA) within a broad range of therapeutic areas including: autoimmune, cardiovascular, infectious, inflammatory and metabolic diseases. Some of the biomarker panels we perform frequently for clients at MuriGenics are below:
- Inflammatory Cytokine/Chemokine Panel
- Endocrine Panel
- Cardiovascular Panel
Flow cytometric analysis allows for the identification and quantification of individual cells in a population with phenotypically discreet properties. Routine assays performed by MuriGenics include:
- Cell Cycle Analysis (PI and Phosphohistone 3 (pH3))
- Apoptosis (Caspase 3/7/8/9)
- PI3 Kinase Signaling
- IgG Quantification
- Custom Assay Solutions
- Detailed Analysis also Available